Preliminary X-Ray Crystallographic Studies of a Lys49-Phospholipase A2 Homologue from Bothrops pirajai Venom Complexed with p-Bromophenacyl Bromide and α-Tocopherol Inhibitors

Author(s): Juliana I. dos Santos , Daniela P. Marchi-Salvador , Carlos A.H. Fernandes , Lucas B. Silveira , Andreimar M. Soares , Marcos R.M. Fontes .

Journal Name: Protein & Peptide Letters

Volume 14 , Issue 7 , 2007

Become EABM
Become Reviewer

Abstract:

PrTX-I, a non-catalytic and myotoxic Lys49-PLA2 from Bothrops pirajai venom has been crystallized alone and in complex with bromophenacyl bromide (BPB), α-tocopherol and α-tocopherol acetate inhibitors. These crystals have shown to diffract X-rays between 2.34 and 1.65 Å resolution. All complexes crystals are isomorphous and belong to the space group P21 whereas native PrTX-I crystals belong to the P3121.

Keywords: Crystallization, X-ray crystallography, Lys49-phospholipase A2, Bothrops pirajai venom, myotoxity, pbromophenacyl bromide, p-BPB, α-tocopherol, vitamin E

Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 14
ISSUE: 7
Year: 2007
Page: [698 - 701]
Pages: 4
DOI: 10.2174/092986607781483796
Price: $58

Article Metrics

PDF: 8