Abstract
The smu.134 gene encodes a putative transcriptional regulator of 217 residues in Streptococcus mutans, a major pathogen for human dental caries. The gene was cloned into expression vector pET28?? and expressed in soluble form in E. coli strain BL21 (DE3) with a His tag at its N-terminus. The recombinant protein SMU.134 was purified to homogeneity in a two step procedure of Ni2+ chelating and size exclusion chromatography. Crystals suitable for X-ray diffraction were obtained by hanging-drop vapor diffusion method and diffracted to 2.6 Å. The crystal belonged to space group P212121, with unit-cell parameters a=55.03 Å, b=80.84 Å, c=107.96 Å.
Keywords: polymerase chain reaction, open reading frames, clostridium beijerincki, sds-page, luria-bertani
Protein & Peptide Letters
Title: Crystallization and Preliminary X-Ray Crystallographic Studies of SMU.134 Protein from Caries Pathogen Streptococcus mutans
Volume: 14 Issue: 5
Author(s): Hua Li, Lan-Fen Li, Xiao-Dong Su, Xiaojun Zhao and Yu-He Liang
Affiliation:
Keywords: polymerase chain reaction, open reading frames, clostridium beijerincki, sds-page, luria-bertani
Abstract: The smu.134 gene encodes a putative transcriptional regulator of 217 residues in Streptococcus mutans, a major pathogen for human dental caries. The gene was cloned into expression vector pET28?? and expressed in soluble form in E. coli strain BL21 (DE3) with a His tag at its N-terminus. The recombinant protein SMU.134 was purified to homogeneity in a two step procedure of Ni2+ chelating and size exclusion chromatography. Crystals suitable for X-ray diffraction were obtained by hanging-drop vapor diffusion method and diffracted to 2.6 Å. The crystal belonged to space group P212121, with unit-cell parameters a=55.03 Å, b=80.84 Å, c=107.96 Å.
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Cite this article as:
Li Hua, Li Lan-Fen, Su Xiao-Dong, Zhao Xiaojun and Liang Yu-He, Crystallization and Preliminary X-Ray Crystallographic Studies of SMU.134 Protein from Caries Pathogen Streptococcus mutans, Protein & Peptide Letters 2007; 14 (5) . https://dx.doi.org/10.2174/092986607780782759
DOI https://dx.doi.org/10.2174/092986607780782759 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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