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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

High-Level Bacterial Expression and Purification of Apicomplexan Micronemal Proteins for Structural Studies

Author(s): S. Saouros, T. M.A. Blumenschein, K. Sawmynaden, J. Marchant, T. Koutroukides, B. Liu, P. Simpson, E. P. Carpenter and S. J. Matthews

Volume 14, Issue 5, 2007

Page: [411 - 415] Pages: 5

DOI: 10.2174/092986607780782876

Price: $65

Abstract

The cysteine-rich N-terminal domain of the micronemal adhesive protein MIC1 (MIC1-NT) from Toxoplasma gondii was cloned, expressed in Escherichia coli and purified. MIC1-NT is amenable to structural studies as shown by preliminary NMR and X-ray analysis. Positive results with two further micronemal proteins indicate that our strategy has wider application.

Keywords: toxoplasma gondii, micronemal proteins, cysteine-rich proteins, nmr, x-ray crystallography

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