Crystallization and Preliminary Crystallographic Study of DNA Polymerase from Pyrococcus furiosus

Author(s): Hirokazu Nishida , Maiko Tanabe , Yoshizumi Ishino , Takuji Oyama , Kosuke Morikawa .

Journal Name: Protein & Peptide Letters

Volume 14 , Issue 4 , 2007

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Abstract:

A new member of archaeal DNA polymerase from Pyrococcus furiosus was crystallized. Diffraction data to 3.1 Å of the selenomethionine-derivatized crystal were collected, and preliminary crystallographic study has been completed. The crystal belongs to the space group C2 with unit cell parameters of a = 93.2 Å, b = 124.9 Å, c = 87.7 Å, α = 90 , β = 109.7® , and γ = 90 . Assuming the presence of one molecule in the asymmetric unit, the solvent content of the crystal is estimated to be 54%, corresponding to a Matthews coefficient VM of 2.7Å3 Da-1.

Keywords: DNA polymerase, crystallization, archaea

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Article Details

VOLUME: 14
ISSUE: 4
Year: 2007
Page: [403 - 405]
Pages: 3
DOI: 10.2174/092986607780363853
Price: $58

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