Protein & Peptide Letters

Prof. Ben M. Dunn  
Department of Biochemistry and Molecular Biology
University of Florida
College of Medicine
P.O. Box 100245
Gainesville, FL
USA
Email: bdunn@ufl.edu

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New Hope for the Diagnosis and Therapy of Alzheimers Disease

Author(s): Bernd Kastenholz.

Abstract:

Improperly folded metal cofactor-containing proteins (e.g., copper chaperone for superoxide dismutase, CCS) are believed to play a key role in several protein-misfolding diseases (e.g., Alzheimers disease or Amyotrophic Lateral Sclerosis) because under regular physiological conditions, metallochaperones activate or stabilize the native conformation of important metalloproteins (e.g., superoxide dismutase) in certain cellular processes. For an improved diagnosis and therapy of neurodegenerative diseases, new methodologies have to be developed that enable a well-defined differentiation between properly folded and inactive metalloproteins in clinical samples. In the literature it is reported that different high molecular mass metal-containing proteins were isolated in brain samples from Alzheimers patients and in vegetables by using a 2-dimensional polyacrylamide gel electrophoresis (2-DE) procedure. In the present article, selected results of these studies are scrutinized and compared with some results obtained by a standardized method termed ‘quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC-PAGE)’. Conclusively, QPNC-PAGE is a highly efficient approach used by biochemists to resolve native and denatured metalloproteins (MW 6 - ≥ 200 kDa) in complex protein mixtures.

Keywords: QPNC-PAGE, 2-DE, GPC, NMR, CCS, Alzheimer's disease, isoelectric point, native conformation, breakthrough method, biofluids

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Article Details

VOLUME: 14
ISSUE: 4
Year: 2007
Page: [389 - 393]
Pages: 5
DOI: 10.2174/092986607780363970
Price: $58