Probing the Effect of Mutations on Cytochrome c Stability

Author(s): Francesco Agueci, Fabio Polticelli, Federica Sinibaldi, Maria Cristina Piro, Roberto Santucci, Laura Fiorucci.

Journal Name: Protein & Peptide Letters

Volume 14 , Issue 4 , 2007

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Although the tertiary structures of mitochondrial cytochromes c (cyts c) seem to be remarkably similar, there are variations in their amino acid sequences, stability and functional properties. GdnHCl-induced unfolding experiments on engineered yeast and horse cyt c were carried out with the aim to to clarify, at molecular level, some aspects concerning the stability of this class of proteins. The results obtained are discussed in the light of the three-dimensional structures of the two proteins.

Keywords: Cytochrome c, guanidinium chloride unfolding, protein stability, circular dichroism, mutagenesis

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Article Details

Year: 2007
Page: [335 - 339]
Pages: 5
DOI: 10.2174/092986607780363989
Price: $58

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