Reevaluating the Capability of Taq DNA Polymerase: Long PCR Amplification

Author(s): Hayan Lee , Kyung-Nam Kim , Young Kee Chae .

Journal Name: Protein & Peptide Letters

Volume 14 , Issue 4 , 2007

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Abstract:

We tested the ability of Taq DNA polymerase (Taq) to amplify long DNA fragments and showed that, if the conditions were set properly, Taq could successfully perform the “long PCR” up to 24 kb. The conditions include: (1) longer primers, (2) a 2-step cycling, and (3) a “long buffer.” We propose that the most important requirements are the survival rate of Taq at high temperatures and that of the primers against the 5 to 3 exonuclease activity of Taq.

Keywords: Taq DNA polymerase, long amplicons, primers, reaction buffer

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Article Details

VOLUME: 14
ISSUE: 4
Year: 2007
Page: [321 - 323]
Pages: 3
DOI: 10.2174/092986607780363934
Price: $58

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