T-Cell Antigen Receptor Assembly and Cell Surface Expression Is Not Affected by Treatment with T-Cell Antigen Receptor-Alpha Chain Transmembrane Peptide
Nozomu Kurosaka, Andrea Bolte, Marina Ali and Nicholas Manolios
Affiliation: Department of Rheumatology,Westmead Hospital, Westmead, NSW 2145, Australia.
A synthetic peptide termed core peptide (CP), which corresponds to a specific sequence of the TCR-α chain transmembrane domain, is known to inhibit IL-2 production in antigen stimulated T-cells. The molecular mechanism of the TCR inhibition is not known. This study examined the effects of CP on TCR subunit assembly and TCR cell surface expression in vitro. Co-transfection experiments between TCR-α and CD3-δ using COS-7 cells, and the interaction between TCR- and the CD3 proteins in a T-cell line (2B4) were analysed after incubation with CP or its conjugates. Results indicate that CP co-precipitates with CD3-δ and CD3-η in vitro, without any effect on TCR-α /CD3-δ dimerisation or TCR multisubunit assembly and cell surface expression.
Keywords: CD3, peptide, assembly, protein-protein interaction, signalling
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