Combinatorial Synthesis and Directed Evolution Applied to the Production of α-Helix Forming Antimicrobial Peptides Analogues

Author(s): Mariana S. Castro, Eduardo M. Cilli, Wagner Fontes.

Journal Name: Current Protein & Peptide Science

Volume 7 , Issue 6 , 2006

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Abstract:

Antimicrobial peptides (AMPs) are effector molecules of innate immune systems found in different groups of organisms, including microorganisms, plants, insects, amphibians and humans. These peptides exhibit several structural motifs but the most abundant AMPs assume an amphipathic α-helical structure. The α-helix forming antimicrobial peptides are excellent candidates for protein engineering leading to an optimization of their biological activity and target specificity. Nowadays several approaches are available and this review deals with the use of combinatorial synthesis and directed evolution in order to provide a high-throughput source of antimicrobial peptides analogues with enhanced lytic activity and specificity.

Keywords: Antimicrobial peptides, amphipathic α-helix, combinatorial synthesis, directed evolution, high-throughput

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Article Details

VOLUME: 7
ISSUE: 6
Year: 2006
Page: [473 - 478]
Pages: 6
DOI: 10.2174/138920306779025648
Price: $58

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