Protein Expression, Crystallization and Preliminary X-Ray Crystallographic Studies on HSCARG from Homo Sapiens

Author(s): Xueyu Dai, Xiaocheng Gu, Ming Luo, Xiaofeng Zheng.

Journal Name: Protein & Peptide Letters

Volume 13 , Issue 9 , 2006

Become EABM
Become Reviewer


Human HSCARG has been annotated as a possible cancer related protein. Amino acid homology, although at a low percentage, suggested that HSCARG contains NmrA domain and might be a member of short chain dehydrogenase reductase superfamily. In order to investigate its structure and function, HSCARG gene has been successfully expressed and purified in E. coli. HSCARG was crystallized and diffracted to a resolution of 2.4 Å on Mar225 CCD Detector at SER-CAT 22BM synchrotron source. The crystals belong to F23 space group, with unit cell parameters a=b=c=223.30Å, α=β=γ=90°. There are two molecules per asymmetry unit.

Keywords: Homo sapiens, HSCARG, protein crystallography

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2006
Page: [955 - 957]
Pages: 3
DOI: 10.2174/092986606778256135
Price: $58