Crystallization and Preliminary X-Ray Analysis of the Catalytic Domain of Chitinase D from Bacillus circulans WL-12

Author(s): Yuichiro Kezuka, Kenji Bando, Hajime Kobayashi, Yasuo Yonou, Toshiya Sato, Takeshi Watanabe, Takamasa Nonaka.

Journal Name: Protein & Peptide Letters

Volume 13 , Issue 9 , 2006

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Abstract:

We report here on crystallization and preliminary X-ray analysis of the catalytic domain of chitinase D from Bacillus circulans WL-12. The native crystals of this domain were found to belong to the orthorhombic space group P212121. To elucidate the structure of the catalytic domain by the multiple isomorphous replacement method, 30 kinds of derivatized crystals were prepared by soaking the native crystals into a mother liquor containing salts of heavy metal atoms. Difference Patterson maps calculated for four derivatives showed strong peaks in the Harker sections.

Keywords: chitinase D, catalytic domain, Bacillus circulans WL-12, crystallization, preliminary X-ray analysis

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Article Details

VOLUME: 13
ISSUE: 9
Year: 2006
Page: [951 - 954]
Pages: 4
DOI: 10.2174/092986606778256108
Price: $58

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