Polydivinylbenzene Hollow Nanospheres as an Effective Fluorescent Sensing Platform for DNA Detection
In this communication, we demonstrate the preparation of polydivinylbenzene hollow nanospheres (PDBHNSs) and their use as an effective sensing platform for fluorescence-enhanced DNA detection for the first time. The DNA detection is accomplished by the following two steps: (1) PDBHNS adsorbs and quenches a dye-labeled single-stranded DNA (ssDNA) probe; (2) The subsequent hybridization of the probe with its target produces a double-stranded DNA (dsDNA) which detaches from PDBHNS, leading to recovery of dye fluorescence. The present system shows a detection limit as low as 5 nM and has a high selectivity down to single-base mismatch.
Keywords: Polydivinylbenzene, hollow nanosphere, fluorescence, DNA detection, pathogen detection, biomedical research, hybridization
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