The Effects of Montmorillonite and Bamboo Vinegar on Porcine Reproductive and Respiratory Syndrome Virus
Early last year, Severe Acute Respiratory Syndrome (termed as SARS) heavily hit the entire Asia and caused a great panic all over the world. A number of agents with different formula have been developed to curb the epidemics. Bamboo vinegar (termed as Bv) is one of the effective agents to de-activate the bacteria. However, bamboo vinegar cannot stay longer (less than 15 min) on the spraying surface due to easy evaporation. In the study, we attempt to prove the ability of bamboo vinegar on virus deactivation and extend the function of bamboo vinegar for longer time. The special characteristics of montmorillonite and bamboo vinegar (termed as M+Bv) are utilized to adsorb virus and deactivate the virus from cell infection. The SARS virus is highly infective and hard to acquire, so we turn to the porcine reproductive and respiratory syndrome (PRRS) virus. The PRRS virus and SARS virus both belong to Nidovirales, and both are RNA viruses with envelope, having similar virus character.
According to the results of electrophoresis diagram of reverse transcription polymerase chain reaction (RT-PCR) product proves that montmorillonite can adsorb PRRS virus and montmorillonite concentration of 25 mg/ml can totally adsorb 5 × 105 TCID50/ml PRRS virus. Bamboo vinegar has a good ability to deactivate PRRS virus and protect the MARC-145 cell from infection. The ability of bamboo vinegar to deactivate virus can be enhanced by montmorillonite. It is proven that co-cultures of M+Bv with virus (termed as M+Bv+V) have good result on PRRS virus deactivation and can extend the ability for 2 weeks or even longer. All the results can be proven by RTPCR, immunofluorescent assay, and transmission electron microscopy. PRRS virus belongs to the same category with SARS virus. The composite of montmorillonite and bamboo vinegar should have a great potential against SARS epidemics.
Keywords: Bamboo vinegar, montmorillonite, porcine reproductive and respiratory syndrome virus, SARS, reverse transcription polymerase chain reaction
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