Fabricating Gradient Hydrogel Scaffolds for 3D Cell Culture

Author(s): Kaushik Chatterjee, Marian F. Young, Carl G. Simon.

Journal Name: Combinatorial Chemistry & High Throughput Screening

Volume 14 , Issue 4 , 2011

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Abstract:

Optimizing cell-material interactions is critical for maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties. Previous CHT platforms in tissue engineering have involved a two-dimensional (2D) cell culture format where cells were cultured on material surfaces. However, these platforms are inadequate to predict cellular response in a three-dimensional (3D) tissue scaffold. We have developed a simple CHT platform to screen cell-material interactions in 3D culture format that can be applied to screen hydrogel scaffolds. Herein we provide detailed instructions on a method to prepare gradients in elastic modulus of photopolymerizable hydrogels.

Keywords: Combinatorial methods, high-throughput screening, hydrogels, elastic modulus, gradients, osteoblast, tissue engineering, 3D Cell Culture, drug discovery, embryonic stem cells, Cell Encapsulation

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Article Details

VOLUME: 14
ISSUE: 4
Year: 2011
Page: [227 - 236]
Pages: 10
DOI: 10.2174/138620711795222455
Price: $58

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