Current Organic Chemistry

Atta-ur-Rahman , FRS
Honorary Life Fellow
Kings College
University of Cambridge
Cambridge
UK

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Microcantilever Biosensors: Probing Biomolecular Interactions at the Nanoscale

Author(s): Dongsheng Liu, Wenmiao Shu.

Abstract:

Microcantilever based biosensors have attracted much attention as a label-free, real-time and highly sensitive approach to the detection of biomolecules. When specific biomolecular interactions occur between a receptor immobilized on one side of a cantilever and a target analyte in solution, a mechanical bending of the cantilever results due to a change in surface stress, thereby translating biochemical interactions into a concentration-dependent nanomechanical response of the microcantilevers. In this review, we present a general overview of the operation principles, fabrication and preparation of microcantilever biosensors and discuss their uses for a wide range of biosensing applications. The focus of the review is given to probing the biomolecular interactions at the solid-liquid interface by measuring the cantilever bending. In addition to the label-free detection of DNA, protein and cells, the emphasis is also made to discuss about the new development of microcantilever biosensors for label-free probing nanoscale conformational changes of DNA, protein and polymers, and real-time detection of nanomechanical forces from living cells. Finally, the outlook for future development work and challenges is also discussed.

Keywords: Microcantilever biosensors, molecular interactions, conformational changes, microarrays, ELISAs, atomic force microscopy (AFM), label-free biosensor, microfabrication, Device Fabrication, AFM, Surface Functionalization, BIOSENSING APPLICATIONS, DNA hybridization reaction, single nucleo-tide polymorphisms (SNPs),, prostate-specific antigen (PSA), human serum albumin, human plasminogen, CDK2, drug vancomycin, mucopetide analogues, piezoelectric cantilever

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Article Details

VOLUME: 15
ISSUE: 4
Year: 2011
Page: [477 - 485]
Pages: 9
DOI: 10.2174/138527211794474519
Price: $58