Novel Small-Molecule Inhibitors of Arylamine N-Acetyltransferases: Drug Discovery by High Throughput Screening

Author(s): Isaac M. Westwood , Akane Kawamura , Angela J. Russell , James Sandy , Stephen G. Davies , Edith Sim .

Journal Name: Combinatorial Chemistry & High Throughput Screening

Volume 14 , Issue 2 , 2011

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Abstract:

Arylamine N-acetyltransferases (NATs) are a family of enzymes found in eukaryotes and prokaryotes. While the precise endogenous function of NAT remains unknown for most organisms, recent evidence has shown that the expression of human NAT1 is up-regulated in estrogen receptor positive breast cancer. Additionally, NAT in mycobacteria is required for mycobacterial cell wall biosynthesis and survival of the organisms within macrophage. It is therefore important to develop small molecule inhibitors of NATs as molecular tools to study the function of NATs in various organisms. Such inhibitors may also prove useful in future drug design, for example in the development of anti tubercular agents. We describe a high throughput screen of a proprietary library of 5016 drug-like compounds against three prokaryotic NAT enzymes and two eukaryotic NAT enzymes.

Keywords: Arylamine N-acetyltransferases, NATs, high throughput screening, drug discovery, Amycolatopsis mediterranei, Mycobacterium bovis, Mycobacterium tuberculosis, TBNAT, Salmonella, STNAT, Mycobacterium smegmatis, MSNAT, Pseudomonas aeruginosa, PANAT, Mesorhizobium loti, MLNAT, Mycobacterium marinum, MMNAT, Tecan Sunrise plate reader, basic median-polish mode, Ellman's reagent, pABA, NMR

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Article Details

VOLUME: 14
ISSUE: 2
Year: 2011
Page: [117 - 124]
Pages: 8
DOI: 10.2174/138620711794474051
Price: $58

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