Abstract
Natural products (NPs) are combinatorial chemical libraries with diversities in chemical structures and pharmacological activities. Screening active compounds is in many cases an important factor in drug discovery. It was not easy to screen out the bioactive compounds from complex extracts consisting of many NPs. Development of rapid, effective and accurate methods is in high demand. During last decades, mass spectrometry (MS)-based strategies, combining isolation, structures, and bioactivity in a single run, were programmed in the NPs screening. The current article reviews different assay formats and applications of MS-based methods for screening of active NPs. This review is divided into three sections based on methods classification. The first part introduces binding-based screening methods that directly assess the binding characteristics of a candidate molecule to its target. The second part describes function-based screening methods that monitor the functional output of a target-dependent biochemical reaction. The third part briefly discusses serum pharmacochemistry-based screening methods that analyze absorbed components and metabolites in plasma after oral administration of NPs extracts.
Keywords: Natural products, mass spectrometry, binding-based screening, function-based screening, serum pharmacochemistry, throughput screening, HTS, TOF, serum pharmacochemistry-based screening, NP-based drug screening., chromatogram, CPDBD, Radix Angelica Sinensis, DAD-ELSD, ESI, Panax notoginseng, Zedoariae rhizoma, ESI-FTICR-MS, multi-target affinity/specificity screening, Streptomyces rimosus, size exclusion chromatography, SEC, MALDI, xanthohumol, Ultrafiltration-MS, Lonicera japonica, Microdialysis-MS, Frontal Affinity Chromatography, Phyllanthus urinaria L, Two-Dimensional Turbulent Flow Chromatography (2D-TFC)-MS, Enzyme Inhibitor Screening, Huperzia serrata, HPLC, Antioxidant Screening, Angelica sinensis
Combinatorial Chemistry & High Throughput Screening
Title: Mass Spectrometry-Based Strategies for Screening of Bioactive Natural Products
Volume: 14 Issue: 2
Author(s): Gui-Zhong Xin, Jian-Liang Zhou, Lian-Wen Qi and Ping Li
Affiliation:
Keywords: Natural products, mass spectrometry, binding-based screening, function-based screening, serum pharmacochemistry, throughput screening, HTS, TOF, serum pharmacochemistry-based screening, NP-based drug screening., chromatogram, CPDBD, Radix Angelica Sinensis, DAD-ELSD, ESI, Panax notoginseng, Zedoariae rhizoma, ESI-FTICR-MS, multi-target affinity/specificity screening, Streptomyces rimosus, size exclusion chromatography, SEC, MALDI, xanthohumol, Ultrafiltration-MS, Lonicera japonica, Microdialysis-MS, Frontal Affinity Chromatography, Phyllanthus urinaria L, Two-Dimensional Turbulent Flow Chromatography (2D-TFC)-MS, Enzyme Inhibitor Screening, Huperzia serrata, HPLC, Antioxidant Screening, Angelica sinensis
Abstract: Natural products (NPs) are combinatorial chemical libraries with diversities in chemical structures and pharmacological activities. Screening active compounds is in many cases an important factor in drug discovery. It was not easy to screen out the bioactive compounds from complex extracts consisting of many NPs. Development of rapid, effective and accurate methods is in high demand. During last decades, mass spectrometry (MS)-based strategies, combining isolation, structures, and bioactivity in a single run, were programmed in the NPs screening. The current article reviews different assay formats and applications of MS-based methods for screening of active NPs. This review is divided into three sections based on methods classification. The first part introduces binding-based screening methods that directly assess the binding characteristics of a candidate molecule to its target. The second part describes function-based screening methods that monitor the functional output of a target-dependent biochemical reaction. The third part briefly discusses serum pharmacochemistry-based screening methods that analyze absorbed components and metabolites in plasma after oral administration of NPs extracts.
Export Options
About this article
Cite this article as:
Xin Gui-Zhong, Zhou Jian-Liang, Qi Lian-Wen and Li Ping, Mass Spectrometry-Based Strategies for Screening of Bioactive Natural Products, Combinatorial Chemistry & High Throughput Screening 2011; 14 (2) . https://dx.doi.org/10.2174/138620711794474060
DOI https://dx.doi.org/10.2174/138620711794474060 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
Call for Papers in Thematic Issues
Artificial Intelligence Methods for Biomedical, Biochemical and Bioinformatics Problems
Recently, a large number of technologies based on artificial intelligence have been developed and applied to solve a diverse range of problems in the areas of biomedical, biochemical and bioinformatics problems. By utilizing powerful computing resources and massive amounts of data, methods based on artificial intelligence can significantly improve the ...read more
Eco-friendly Agents for Biological Control of Pathogenic Diseases
The discovery of an alternative biological approach to disease management includes work on medicinal products derived from natural sources as a starting point for the development of eco-friendly agents for these diseases and the injuries they cause, as well as reducing human contact with hazardous chemicals and their residues. We ...read more
Emerging trends in diseases mechanisms, noble drug targets and therapeutic strategies: focus on immunological and inflammatory disorders
Recently infectious and inflammatory diseases have been a key concern worldwide due to tremendous morbidity and mortality world Wide. Recent, nCOVID-9 pandemic is a good example for the emerging infectious disease outbreak. The world is facing many emerging and re-emerging diseases out breaks at present however, there is huge lack ...read more
Exploring Spectral Graph Theory in Combinatorial Chemistry
Scope of the Thematic Issue: Combinatorial chemistry involves the synthesis and analysis of a large number of diverse compounds simultaneously. Traditional methods rely on brute force experimentation, which can be time-consuming and resource-intensive. Spectral Graph Theory, a branch of mathematics dealing with the properties of graphs in relation to the ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Overview on Anticancer Drug Design and Development
Current Medicinal Chemistry Phospholipase D Inhibition: Beneficial and Harmful Consequences for a Double-Dealer Enzyme
Current Enzyme Inhibition Strategies for Targeting Lentiviral Vectors
Current Gene Therapy Resveratrol, a Phytochemical Inducer of Multiple Cell Death Pathways: Apoptosis, Autophagy and Mitotic Catastrophe
Current Medicinal Chemistry Nanoparticles Improve Biological Functions of Phthalocyanine Photosensitizers Used for Photodynamic Therapy
Current Drug Metabolism Regulatory T Cells: Major Players in the Tumor Microenvironment
Current Pharmaceutical Design Ruthenium Complexes: An Emerging Ground to the Development of Metallopharmaceuticals for Cancer Therapy
Mini-Reviews in Medicinal Chemistry ROS1 Kinase Inhibitors for Molecular-Targeted Therapies
Current Medicinal Chemistry Single Nucleotide Polymorphisms as the Efficient Prognostic Markers in Breast Cancer
Current Cancer Drug Targets Targeted Therapies in Hepatocellular Carcinoma
Current Medicinal Chemistry Curcumin: the Yellow Molecule with Pleiotropic Biological Effects
Letters in Drug Design & Discovery Vitamin D and Breast Cancer Incidence and Outcome
Anti-Cancer Agents in Medicinal Chemistry Regulation of Sulfotransferases by Xenobiotic Receptors
Current Drug Metabolism Genetics of the First Seven Proprotein Convertase Enzymes in Health and Disease
Current Genomics Distinguished Photons: A Review of In Vivo Spectral Fluorescence Imaging in Small Animals
Current Pharmaceutical Biotechnology Optimizing Tumor-Reactive γδT Cells for Antibody-Based Cancer Immunotherapy
Current Molecular Medicine Inflammatory Markers in Coronary Artery Disease: Pathophysiological Mechanisms, Prognostic and Therapeutic Implications
Current Cardiology Reviews Neuroprotective Role of Hypothermia in Hypoxic-ischemic Brain Injury: Combined Therapies using Estrogen
Current Neuropharmacology The Characteristics, Functions and Inhibitors of Three Aminopeptidases Belonging to the M1 Family
Current Protein & Peptide Science Anti-Cancer Properties of Nigella spp. Essential Oils and their Major Constituents, Thymoquinone and β-Elemene
Current Clinical Pharmacology