Abstract
Nucleic acids transfer has been steadily improving over the years and is slowly starting to fulfill its long awaited promises. In the beginning, viral approaches raised strong safety concerns that are now answered by various nonviral techniques. Among the physical approaches developed, nucleic acids electrotransfer is probably the one with the highest momentum. Here we review the present knowledge on the mechanistic and practical aspects of in vivo nucleic acids electrotransfer. For each step of this procedure we present different strategies that are used, with their advantages and drawbacks. As we report here, practical solutions have been found to overcome each limiting step in the procedure and to improve its outcome. Some crucial issues are beyond the application of the electric pulses itself, like the administration (i.e., in almost all of the cases, the injection) of the nucleic acids to the tissue or the body. High efficiency and safety are at reach if all the present knowledge and strategies are put to use. Electrotransfer is now a mature technique as proven by the fact that clinical trials using nucleic acids electrotransfer have already started within the past few years.
Keywords: DNA injection, electric pulses, electropermeabilization, electrophoresis, electroporation, minicircle, plasmid
Current Gene Therapy
Title: Nucleic Acids Electrotransfer In Vivo: Mechanisms and Practical Aspects
Volume: 10 Issue: 4
Author(s): Franck M. Andre and Lluis M. Mir
Affiliation:
Keywords: DNA injection, electric pulses, electropermeabilization, electrophoresis, electroporation, minicircle, plasmid
Abstract: Nucleic acids transfer has been steadily improving over the years and is slowly starting to fulfill its long awaited promises. In the beginning, viral approaches raised strong safety concerns that are now answered by various nonviral techniques. Among the physical approaches developed, nucleic acids electrotransfer is probably the one with the highest momentum. Here we review the present knowledge on the mechanistic and practical aspects of in vivo nucleic acids electrotransfer. For each step of this procedure we present different strategies that are used, with their advantages and drawbacks. As we report here, practical solutions have been found to overcome each limiting step in the procedure and to improve its outcome. Some crucial issues are beyond the application of the electric pulses itself, like the administration (i.e., in almost all of the cases, the injection) of the nucleic acids to the tissue or the body. High efficiency and safety are at reach if all the present knowledge and strategies are put to use. Electrotransfer is now a mature technique as proven by the fact that clinical trials using nucleic acids electrotransfer have already started within the past few years.
Export Options
About this article
Cite this article as:
M. Andre Franck and M. Mir Lluis, Nucleic Acids Electrotransfer In Vivo: Mechanisms and Practical Aspects, Current Gene Therapy 2010; 10 (4) . https://dx.doi.org/10.2174/156652310791823380
DOI https://dx.doi.org/10.2174/156652310791823380 |
Print ISSN 1566-5232 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5631 |
Call for Papers in Thematic Issues
Programmed Cell Death Genes in Oncology: Pioneering Therapeutic and Diagnostic Frontiers (BMS-CGT-2024-HT-45)
Programmed Cell Death (PCD) is recognized as a pivotal biological mechanism with far-reaching effects in the realm of cancer therapy. This complex process encompasses a variety of cell death modalities, including apoptosis, autophagic cell death, pyroptosis, and ferroptosis, each of which contributes to the intricate landscape of cancer development and ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Targeting mTOR Pathways in Human Malignancies
Current Pharmaceutical Design Stimuli-Responsive Nanoparticles for siRNA Delivery
Current Pharmaceutical Design G Protein-Coupled Receptors and their Signaling Pathways: Classical Therapeutical Targets Susceptible to Novel Therapeutic Concepts
Current Pharmaceutical Design Revisiting the ABCs of Multidrug Resistance in Cancer Chemotherapy
Current Pharmaceutical Biotechnology Nano-Neurotherapeutics (NNTs): An Emergent and Multifaceted Tool for CNS Disorders
Current Drug Metabolism Pharmacogenomics of Cytochrome P450 Enzymes in Tumours
Current Pharmacogenomics Effects of Highly Active Antiretroviral Therapy on HIV-1-Associated Oral Complications
Current HIV Research Anthracyclines Still Prove Effective in Anticancer Therapy
Mini-Reviews in Medicinal Chemistry Biological Agents Involved in Malignant Mesothelioma: Relevance as Biomarkers or Therapeutic Targets
Current Cancer Drug Targets Modulation of Tumour-Related Signaling Pathways by Natural Pentacyclic Triterpenoids and their Semisynthetic Derivatives
Current Medicinal Chemistry The Therapeutic Potential of ADAM15
Current Pharmaceutical Design Discovery of Novel Plant Peptides as Strong Inhibitors of Metalloproteinases
Protein & Peptide Letters Triggering PIK3CA Mutations in PI3K/Akt/mTOR Axis: Exploration of Newer Inhibitors and Rational Preventive Strategies
Current Pharmaceutical Design Patent Analysis as a Tool for Research Planning: Study on Natural Based Therapeutics Against Cancer Stem Cells
Recent Patents on Anti-Cancer Drug Discovery Biosystems Engineering of Prokaryotes with Tumor-Killing Capacities
Current Pharmaceutical Design microRNA Biogenesis Pathway as a Therapeutic Target for Human Disease and Cancer
Current Pharmaceutical Design Calcium-calpain Dependent Pathways Regulate Vesiculation in Malignant Breast Cells
Current Cancer Drug Targets Metal Containing Cytostatics and Their Interaction with Cellular Thiol Compounds Causing Chemoresistance
Anti-Cancer Agents in Medicinal Chemistry Sphingolipid Modulation: A Strategy for Cancer Therapy
Anti-Cancer Agents in Medicinal Chemistry Metabolic Response Assessment in Non-Small Cell Lung Cancer Patients after Platinum-Based Therapy: A Preliminary Analysis
Current Medical Imaging