The amino functionality gives important biological activity in pharmaceutical compounds. The formation of chiral amines and amino acids can be accomplished by several chemical routes but enzymatic formation of amines offers many advantages in preparing chiral amino compounds or amination of fragile compounds compared to stoichiometric or catalytic chemical transformations. Biocatalytic routes to amines primarily use enzymes of the transaminase class (also known as aminotransferases) which transfer the amino function from a donor organic compound to a ketone or aldehyde acceptor. Although known since 1937, the transamination reaction experiences renewed interest due to the advances in biochemistry and molecular biology and the excellent selectivity of biocatalysts. Other enzymes that have been used to synthesize chiral amines are from the phenylalanine ammonia lyase class that use ammonia as the amino source. The use of recombinant enzymes for the biocatalytic preparation of amines is expanding at a great rate and the range of enzymes revealed in DNA sequence databases is of the order of tens of thousands. Since a large number of substrates like ketones, hydroxyketones and ketoacids can be made by chemical synthesis, the growing toolbox of α- , β-, γ-, δ-, ε- and ω-transaminases enable the synthesis of various new chemical entities by biocatalytic amination reactions. In order to simplify the isolation and purification of the product, it is useful to drive the amination reaction to completion. The biocatalytic processes that have been developed show different strategies of overcoming the kinetic limitations of the transaminase reactions and show how some enzymes have been used in processes to make large quantities of chiral compounds with amino functionalities.
Keywords: Biocatalytic Transamination, transaminase, aminotransferase, enzyme, biocatalysis, biocatalytic process, phenylalanine ammonia lyase, amino acid, screening, bioprocess, aldehyde, ketone, biotransformation, Transamination, process, phenylalanine, amino acids, screenin, reductive amination, dialkylaluminium amides, C-N bonds, NaCNBH3, GABA, NADPH, L-glutamic acid, aminotransferases, chiral amines, CLUSTAL, (SAT), L-glutamate, PLP, D-Alanine TAm, Serine TAm, Phosphoserine TAm, TDP-4-amino-4,6-dideoxy- D-glucose TAm, transaminases, PAL, (TAL), HAL, Rhodococcus species, Mesorhizobium sp, L-erythrulose, Cambrex, total gene synthesis, NMR binding screening, (BiCE)
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