Urea-Gradient Protein Refolding in Size Exclusion Chromatography

Author(s): Chaozhan Wang, Yan Cheng.

Journal Name: Current Pharmaceutical Biotechnology

Volume 11 , Issue 3 , 2010

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Protein refolding using urea gradient size exclusion chromatography (UGSEC) is a new version of conventional SEC refolding, which incorporates urea gradient into the SEC. Operating factors, mainly the urea gradient length and the final urea concentration in the gradient, are discussed in detail. Recent applications of UGSEC, including refolding of recombinant human granulocyte colony stimulating factor by UGSEC, is shown as an example.

Keywords: Urea gradient, size exclusion chromatography, protein refolding, recombinant human granulocyte colony stimulating factor

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Article Details

Year: 2010
Page: [289 - 292]
Pages: 4
DOI: 10.2174/138920110791112004
Price: $58

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