Current Pharmaceutical Biotechnology

Zeno Foldes-Papp
Visiting Professor of Medical Biochemistry
HELIOS Clinical Center of Emergency Medicine
Department for Internal Medicine
Alte-Koelner-Strasse 9
D-51688 Koeln-Wipperfuerth
Germany

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Step-Wise Refolding of Recombinant Proteins

Author(s): Kouhei Tsumoto, Tsutomu Arakawa and Linda Chen

Affiliation: Department of Medical Genome Sciences, Graduate School of Engineering, The University of Tokyo; 5-1-5 Kashiwanoha, Kashiwa 277-8562, Japan.

Keywords: Step-wise refolding, arginine, aggregation, antibody fragment, dialysis

Abstract:

Protein refolding is still on trial-and-error basis. Here we describe step-wise dialysis refolding, in which denaturant concentration is altered in step-wise fashion. This technology controls the folding pathway by adjusting the concentrations of the denaturant and other solvent additives to induce sequential folding or disulfide formation.

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Article Details

VOLUME: 11
ISSUE: 3
Page: [285 - 288]
Pages: 4
DOI: 10.2174/138920110791111979