Step-Wise Refolding of Recombinant Proteins
Kouhei Tsumoto, Tsutomu Arakawa and Linda Chen
Affiliation: Department of Medical Genome Sciences, Graduate School of Engineering, The University of Tokyo; 5-1-5 Kashiwanoha, Kashiwa 277-8562, Japan.
Keywords: Step-wise refolding, arginine, aggregation, antibody fragment, dialysis
Protein refolding is still on trial-and-error basis. Here we describe step-wise dialysis refolding, in which denaturant concentration is altered in step-wise fashion. This technology controls the folding pathway by adjusting the concentrations of the denaturant and other solvent additives to induce sequential folding or disulfide formation.
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