Current Pharmaceutical Biotechnology

Davide Prosperi
Department of Biotechnology and Bioscience
University of Milan Bicocca
Milano
Italy

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A Highly Controllable Reconstituted Cell-Free System -a Breakthrough in Protein Synthesis Research

Author(s): Hiroyuki Ohashi, Takashi Kanamori, Yoshihiro Shimizu, Takuya Ueda.

Abstract:

The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.

Keywords: Cell-free protein synthesis, in vitro translation, chaperone, disulfide bond formation, in vitro selection, unnatural amino acid

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Article Details

VOLUME: 11
ISSUE: 3
Year: 2010
Page: [267 - 271]
Pages: 5
DOI: 10.2174/138920110791111889
Price: $58