A Highly Controllable Reconstituted Cell-Free System -a Breakthrough in Protein Synthesis Research
Hiroyuki Ohashi, Takashi Kanamori, Yoshihiro Shimizu and Takuya Ueda
Affiliation: Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, FSB-401, 5-1-5, Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
Keywords: Cell-free protein synthesis, in vitro translation, chaperone, disulfide bond formation, in vitro selection, unnatural amino acid
The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.
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