Recombinant Expression in Moderate Halophiles

Author(s): Masao Tokunaga, Tsutomu Arakawa, Hiroko Tokunaga.

Journal Name: Current Pharmaceutical Biotechnology

Volume 11 , Issue 3 , 2010

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Abstract:

A novel expression of recombinant proteins was developed using moderate halophiles that accumulate osmolytes and hence provide cytoplasmic environments where osmolyte-driven folding can take place. Promoters and selection marker were developed for high expression of foreign proteins. Examples are given for expression of bacterial nucleoside diphosphate kinase and human serine racemase.

Keywords: Soluble expression, moderate halophile, compatible solute, racemase, kinase

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Article Details

VOLUME: 11
ISSUE: 3
Year: 2010
Page: [259 - 266]
Pages: 8
DOI: 10.2174/138920110791112022
Price: $58

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