Abstract
While the Baculovirus Expression Vector System (BEVS) mainly uses insect cell lines, such as Sf9 cells, the robust high expression system using silkworm has also been developed. We have further improved technologies for enhancement of virus recombination, reduction of proteolytic degradation and aggregation, and more reliable promoters. These developments made it possible to achieve high and soluble expression of recombinant proteins. We review here such technology developments, advantage of using silkworm and some example applications. There are areas where this technology can be further improved as implicated in the end.
Keywords: Baculovirus, silkworm, polyhedrin, cathepsin-like protease, vp39, Dock-tag
Current Pharmaceutical Biotechnology
Title: Silkworm as a Host of Baculovirus Expression
Volume: 11 Issue: 3
Author(s): Akihiro Usami, Takeo Suzuki, Hidekazu Nagaya, Hiroki Kaki and Seiji Ishiyama
Affiliation:
Keywords: Baculovirus, silkworm, polyhedrin, cathepsin-like protease, vp39, Dock-tag
Abstract: While the Baculovirus Expression Vector System (BEVS) mainly uses insect cell lines, such as Sf9 cells, the robust high expression system using silkworm has also been developed. We have further improved technologies for enhancement of virus recombination, reduction of proteolytic degradation and aggregation, and more reliable promoters. These developments made it possible to achieve high and soluble expression of recombinant proteins. We review here such technology developments, advantage of using silkworm and some example applications. There are areas where this technology can be further improved as implicated in the end.
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Cite this article as:
Usami Akihiro, Suzuki Takeo, Nagaya Hidekazu, Kaki Hiroki and Ishiyama Seiji, Silkworm as a Host of Baculovirus Expression, Current Pharmaceutical Biotechnology 2010; 11 (3) . https://dx.doi.org/10.2174/138920110791112013
DOI https://dx.doi.org/10.2174/138920110791112013 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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