Protein-protein interaction network represents an important aspect of systems biology. The understanding of the plant proteinprotein interaction network and interactome will provide crucial insights into the regulation of plant developmental, physiological, and pathological processes. In this review, we will first define the concept of plant interactome and the protein-protein interaction network. The significance of the plant interactome study will be discussed. We will then compare the pros and cons for different strategies for interactome mapping including yeast two-hybrid system (Y2H), affinity purification mass spectrometry (AP-MS), bimolecular fluorescence complementation (BiFC), and in silico prediction. The application of these platforms on specific plant biology questions will be further discussed. The recent advancements revealed the great potential for plant protein-protein interaction network and interactome to elucidate molecular mechanisms for signal transduction, stress responses, cell cycle control, pattern formation, and others. Mapping the plant interactome in model species will provide important guideline for the future study of plant biology.