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Nano-Mechanics and Membrane Receptor Mapping of CD8 + T Cells (Supplementry Material)

Author(s): Yangzhe Wu, Yi Hu, Hongsong Lu, Jiye Cai, Xianhui He, Hongxia Zhao.

Abstract:

The investigation of nano-mechanical properties and nano-architectures of CD8 + T cells activated in vitro will help further interpret the immune response/recognition of these cells at nanoscale resolution. In this work, the local biophysical properties of human peripheral blood CD8 + T cells were obtained, revealing the adhesion force of membrane was increased after CD8 + T cells were stimulated with phorbol dibutyrate plus ionomycin, though the measured local stiffness for resting and activated cells was similar. The 3-D distribution pattern and grayscale map together revealed that membrane receptors (CD8 and activation marker CD69) distributed non-uniformly on the membrane and were shown as micro-, nano-scale clusters or domains. This work describes a relatively simple approach for exploiting the distribution pattern of receptor molecules on a single T cell surface.

Keywords: Force spectroscopy, molecule recognition, receptor mapping, nano-mechanics, CD8 + T cell

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Article Details

VOLUME: 5
ISSUE: 4
Year: 2009
Page: [465 - 469]
Pages: 5
DOI: 10.2174/157341309789378096
Price: $58