Abstract
Mass spectrometry can be used to obtain information about all levels of protein structure. To gain access to the conformation information found in the tertiary and quaternary structure, various labeling methods have been developed. These methods convert structural information into mass differences that can be observed with high-resolution protein/ peptide mass spectrometry. Three methods are reviewed here: hydrogen/deuterium exchange, covalent modification (also called chemical modification) and hydroxyl radical footprinting. The general implementation of these methods is described and comparisons are made between the methods.
Keywords: Hydrogen exchange, Mass spectrometry, Covalent labeling, Hydroxyl radical footprinting
Current Analytical Chemistry
Title: Isotope Exchange and Covalent Modification Strategies for Studying Protein Structure and Function
Volume: 5 Issue: 2
Author(s): Shugui Chen and John R. Engen
Affiliation:
Keywords: Hydrogen exchange, Mass spectrometry, Covalent labeling, Hydroxyl radical footprinting
Abstract: Mass spectrometry can be used to obtain information about all levels of protein structure. To gain access to the conformation information found in the tertiary and quaternary structure, various labeling methods have been developed. These methods convert structural information into mass differences that can be observed with high-resolution protein/ peptide mass spectrometry. Three methods are reviewed here: hydrogen/deuterium exchange, covalent modification (also called chemical modification) and hydroxyl radical footprinting. The general implementation of these methods is described and comparisons are made between the methods.
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Cite this article as:
Chen Shugui and Engen R. John, Isotope Exchange and Covalent Modification Strategies for Studying Protein Structure and Function, Current Analytical Chemistry 2009; 5 (2) . https://dx.doi.org/10.2174/157341109787846144
DOI https://dx.doi.org/10.2174/157341109787846144 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
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