Login

Journal Image
Current Analytical Chemistry
ISSN (Print): 1573-4110
ISSN (Online): 1875-6727
VOLUME: 5
ISSUE: 2
DOI: 10.2174/157341109787846117      Price:  $58









Bottom-Up Proteomics

img
Author(s): Andrea Armirotti
Pages 116-130 (15)
Abstract:
In this work, the “bottom-up” protein identification process is described, from sample digestion to the final database search result. Both MALDI and LC-MS approaches to the identification issue are illustrated. The state-of-the-art of these technique is outlined along with several recent applications, such as MSn data acquisition and chemical derivatization of peptides.
Keywords:
Proteomics, BOTTOM UP PROCESS, MALDI, LC-MS
Affiliation:
Department of Experimental Medicine, Biochemistry Section, University of Genoa, Viale Benedetto XV, 7 Genova, Italy.