In this work, the “bottom-up” protein identification process is described, from sample digestion to the final database search result. Both MALDI and LC-MS approaches to the identification issue are illustrated. The state-of-the-art of these technique is outlined along with several recent applications, such as MSn data acquisition and chemical derivatization of peptides.
Proteomics, BOTTOM UP PROCESS, MALDI, LC-MS
Department of Experimental Medicine, Biochemistry Section, University of Genoa, Viale Benedetto XV, 7 Genova, Italy.