Current Analytical Chemistry

Samuel Achilefu
Washington University
St. Louis, MO
USA
Email: cac@benthamscience.org

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Bottom-Up Proteomics

Author(s): Andrea Armirotti

Affiliation: Department of Experimental Medicine, Biochemistry Section, University of Genoa, Viale Benedetto XV, 7 Genova, Italy.

Abstract:

In this work, the “bottom-up” protein identification process is described, from sample digestion to the final database search result. Both MALDI and LC-MS approaches to the identification issue are illustrated. The state-of-the-art of these technique is outlined along with several recent applications, such as MSn data acquisition and chemical derivatization of peptides.

Keywords: Proteomics, BOTTOM UP PROCESS, MALDI, LC-MS

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Article Details

VOLUME: 5
ISSUE: 2
Page: [116 - 130]
Pages: 15
DOI: 10.2174/157341109787846117
Price: $58