Bottom-Up Proteomics

Author(s): Andrea Armirotti.

Journal Name: Current Analytical Chemistry

Volume 5 , Issue 2 , 2009

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Abstract:

In this work, the “bottom-up” protein identification process is described, from sample digestion to the final database search result. Both MALDI and LC-MS approaches to the identification issue are illustrated. The state-of-the-art of these technique is outlined along with several recent applications, such as MSn data acquisition and chemical derivatization of peptides.

Keywords: Proteomics, BOTTOM UP PROCESS, MALDI, LC-MS

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Article Details

VOLUME: 5
ISSUE: 2
Year: 2009
Page: [116 - 130]
Pages: 15
DOI: 10.2174/157341109787846117
Price: $58

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