High Resolution Protein Display by Two-Dimensional Electrophoresis

Author(s): Gert Van den Bergh, Lutgarde Arckens.

Journal Name: Current Analytical Chemistry

Volume 5 , Issue 2 , 2009

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Abstract:

Two-dimensional electrophoresis has, for many years, been the primary workhorse for performing functional proteomics, the large-scale analysis of protein expression differences. Despite its merits, limitations inherent to this technology have been recognized for a long time, ranging from its gel-to-gel variability to its inability to represent several classes of proteins. Recently, however, technical advances in two-dimensional electrophoresis have alleviated several of these drawbacks. Fractionation approaches prior to two-dimensional electrophoresis, e.g. by chromatography, organelle fractionation or Equalizer Beads technology, have increased the number of visible proteins. Fluorescent two-dimensional difference gel electrophoresis has boosted the quantitative aspects of two-dimensional electrophoresis. New protein stains have also enabled the analysis of post-translationally modified proteins. As a result, two-dimensional electrophoresis has been thoroughly modernized, enabling it to remain the preferred method for protein expression analysis in a large number of laboratories. In this review we will give an overview of these technological advances.

Keywords: Two-dimensionalelectrophoresis, Proteomics, Phosphorylation, Glycosylation, 2-D DIGE, Protein prefractionation

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Article Details

VOLUME: 5
ISSUE: 2
Year: 2009
Page: [106 - 115]
Pages: 10
DOI: 10.2174/157341109787846199
Price: $58

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