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Combinatorial Chemistry & High Throughput Screening

Editor-in-Chief

ISSN (Print): 1386-2073
ISSN (Online): 1875-5402

Offline and Online Liquid Chromatography Mass Spectrometry in Quantitative Proteomics

Author(s): Jessica Z. Bereszczak and Francesco L. Brancia

Volume 12, Issue 2, 2009

Page: [185 - 193] Pages: 9

DOI: 10.2174/138620709787315418

Price: $65

Abstract

Hyphenation with liquid chromatography has become indispensable in mass spectrometry-based proteomics. Sample complexity together with the large variations in dynamic range can be only tackled using techniques that isolate and/or concentrate individual components prior to mass spectrometric analysis. In this review the most recent developments in micro/nanoliquid chromatography interfaced with MALDI and electrospray ionisation are discussed. Particular attention is focused on all applications related to quantitative proteomics.

Keywords: Liquid Chromatography, Mass Spectrometry, proteomics, MALDI, electrospray ionisation


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