Structure-Function Relationships of the Non-Lanthionine-Containing Peptide (class II) Bacteriocins Produced by Gram-Positive Bacteria

Author(s): J. Nissen-Meyer, P. Rogne, C. Oppegard, H. S. Haugen, P. E. Kristiansen.

Journal Name:Current Pharmaceutical Biotechnology

Volume 10 , Issue 1 , 2009

Abstract:

This review focuses on the structure and mode-of-action of non-lanthionine-containing peptide bacteriocins produced by Gram-positive bacteria. These bacteriocins may be divided into four groups: (i) the anti-listerial one-peptide pediocin-like bacteriocins that have very similar amino acid sequences, (ii) the two-peptide bacteriocins that consist of two different peptides, (iii) the cyclic bacteriocins, and (iv) the linear non-pediocin-like onepeptide bacteriocins. These bacteriocins are largely cationic, contain 20 to 70 residues, and kill cells through membrane- permeabilization. The pediocin-like bacteriocins are the ones that are best characterized. Upon contact with target membranes, their cationic N-terminal half forms a β-sheet-like structure that binds to the target cell surface, while their more hydrophobic helical-containing C-terminal half penetrates into the hydrophobic core of target-cell membranes and apparently binds to the mannose phosphotransferase permease in a manner that results in membrane leakage. Immunity proteins that protect cells from being killed by pediocin-like bacteriocins bind to the bacteriocin- permease complex and prevent bacteriocin-induced membrane-leakage. Recent structural analyses of two-peptide bacteriocins indicate that they form a helix-helix structure that penetrates into cell membranes. Also these bacteriocins may act by binding to integrated membrane proteins. It is proposed that many membrane-active peptide bacteriocins kill target-cells through basically the same mechanism; the common theme being that a membranepenetrating part of bacteriocins bind to a membrane embedded region of an integrated membrane protein, thereby causing conformational alterations in the protein that in turn lead to membrane-leakage and cell death.

Keywords: Bacteriocins, antimicrobial peptides, immunity proteins, pediocin-like bacteriocins, two-peptide bacteriocins, cyclic bacteriocins

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Article Details

VOLUME: 10
ISSUE: 1
Year: 2009
Page: [19 - 37]
Pages: 19
DOI: 10.2174/138920109787048661