The Chiptip: A Novel Tool for Automated Patch Clamp

Author(s): Andjelko Golubovic, Martina Knirsch, Uwe Czubayko, Alexander Hummer, Albrecht Lepple-Wienhues, Dirck Lassen.

Journal Name: Combinatorial Chemistry & High Throughput Screening

Volume 12 , Issue 1 , 2009

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Abstract:

To facilitate automated patch clamp measurements of ion channels in cells, the development of an all-glass Chiptip pipette is reported that may be combined with the previously described Flip-the-Tip technology. A single measurement requires less than 50 cells, and the addition of drugs for screening can be limited to very low volumes down to 1 μL. This apparatus is suitable for the study small cells, subcellular organelles and bacteria.

Keywords: ligand gated channel, voltage gated channel, membrane protein, glass micropipettes, automated patch clamp, Ion channel

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Article Details

VOLUME: 12
ISSUE: 1
Year: 2009
Page: [73 - 77]
Pages: 5
DOI: 10.2174/138620709787047984

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