Current Drug Targets

Francis J. Castellino
Kleiderer-Pezold Professor of Biochemistry
Director, W.M. Keck Center for Transgene Research
Dean Emeritus, College of Science
230 Raclin-Carmichael Hall, University of Notre Dame
Notre Dame, IN 46556
USA

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Transcriptional Regulatory Networks in Entamoeba histolytica

Author(s): Gretchen M. Ehrenkaufer and Upinder Singh

Affiliation: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, 94305-5107, USA.

Keywords: Microarrays, protozoan parasite, stage conversion, invasion, virulence, gene expression

Abstract:

Expression profiling with microarray technology has revolutionized exploration of transcriptional regulatory networks on a genome-wide scale. This approach has been successfully applied to the study of Entamoeba histolytica, which causes dysentery and liver abscesses and is a leading parasitic cause of death globally. A variety of microarray platforms have been developed for this system including those generated from genomic DNA, long oligonucleotides, and short oligonucleotides. Using these tools researchers have identified parasite genes whose transcript abundance is differentially regulated during stress, host invasion, and stage conversion. Additionally, novel virulence factors have been identified by identifying genes that are highly expressed in virulent but with low expression in non-virulent Entamoeba strains. All combined, these studies have provided new data on molecular aspects of amebic biology, pathogenic potential and stage conversion and provide investigators with the first insights into potential novel drug targets against amebic disease.

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Article Details

VOLUME: 9
ISSUE: 11
Page: [931 - 937]
Pages: 7
DOI: 10.2174/138945008786786109