Cell-Based Assays in Practice: Cell Markers from Autofluorescent Proteins of the GFP-Family

Title: Cell-Based Assays in Practice: Cell Markers from Autofluorescent Proteins of the GFP-Family

Volume: 11 Issue: 8

Author(s): Michael Wolff, Simone Kredel, Jorg Wiedenmann, G. Ulrich Nienhaus and Ralf Heilker

Affiliation:

Keywords: Fluorescent proteins, High Content Screening, fluorescence microscopy, imaging, anthozoan

Abstract: The more recently discovered anthozoan fluorescent proteins (FPs) and the classic Aequorea victoria Green Fluorescent Protein (avGFP) as well as their derivatives have become versatile tools as live cell markers in fluorescence microscopy. In this review, we show the use of these FPs in drug discovery assays. Assay examples are given for the application of FPs in multiplexed imaging, as photosensitizers, as fluorescent timers, as pulse-chase labels and for robotically integrated compound testing. The development of fast microscopic imaging devices has enabled the application of automated fluorescence microscopy combined with image analysis to pharmaceutical high throughput drug discovery assays, generally referred to as High Content Screening (HCS).

Cite this article as:

Wolff Michael, Kredel Simone, Wiedenmann Jorg, Nienhaus Ulrich G. and Heilker Ralf, Cell-Based Assays in Practice: Cell Markers from Autofluorescent Proteins of the GFP-Family, Combinatorial Chemistry & High Throughput Screening 2008; 11 (8) . https://dx.doi.org/10.2174/138620708785739880