Current Medicinal Chemistry

Atta-ur-Rahman, FRS
Honorary Life Fellow
Kings College
University of Cambridge
Cambridge
UK

Back

Advances in Parallel Screening of Drug Candidates

Author(s): Paolo Bergese, Marina Cretich, Claudio Oldani, Giulio Oliviero, Gabriele Di Carlo, Laura E. Depero, Marcella Chiari.

Abstract:

In the hit to lead process, a drug candidate is selected from a set of potential leads by screening its binding with potential targets. This review focuses on the lead identification assays that employ a bio-chemical or bio-physical test to detect molecular recognition events between proteins and small molecules in a parallel format.These tests require either the lead or the target immobilization followed by incubation with the set of potential interaction partners and detection of a signal related to the target-ligand binding. In the first part of the review the different detection strategies amenable for drug screening are discussed. In the second part, a review of immobilization approaches for leads or targets, allowing the parallel screening of arrays of molecules, is presented.

Keywords: Lead, drug, detection, drug discovery, high throughput screening, small molecule microarrays

Order Reprints Order Eprints Rights & PermissionsPrintExport

Article Details

VOLUME: 15
ISSUE: 17
Year: 2008
Page: [1706 - 1719]
Pages: 14
DOI: 10.2174/092986708784872366
Price: $58