Abstract
Previously we have shown that E2 down regulates S-COMT expression. Here the effects of four phthalate esters and 4-(tertoctyl) phenol on the intra-cellular levels of S-COMT and COMT activity were studied in MCF-7 cells as a measure of estrogenic activity of these compounds. The four phthalate esters caused significant reductions in both S-COMT protein and COMT activity levels. These effects were inhibited by the ERα receptor antagonist ICI182780. 4-(tert-octyl)phenol also caused reductions in these parameters, but the effects were not abolished by ICI182780. Assay of S-COMT protein levels represents a simple and convenient method of assessing the estrogenic potential of a compound.
Keywords: COMT, plasticiser, phthalate, endocrine disruption, estrogen
Current Drug Metabolism
Title: Effects of Plasticisers and Related Compounds on the Expression of the Soluble Form of Catechol-O-Methyltransferase in MCF-7 Cells
Volume: 9 Issue: 4
Author(s): P. W.L. Ho, A. C.Y. Chu, K. H.H. Kwok, H. F. Liu, M. H.W. Kung, D. B. Ramsden and S. L. Ho
Affiliation:
Keywords: COMT, plasticiser, phthalate, endocrine disruption, estrogen
Abstract: Previously we have shown that E2 down regulates S-COMT expression. Here the effects of four phthalate esters and 4-(tertoctyl) phenol on the intra-cellular levels of S-COMT and COMT activity were studied in MCF-7 cells as a measure of estrogenic activity of these compounds. The four phthalate esters caused significant reductions in both S-COMT protein and COMT activity levels. These effects were inhibited by the ERα receptor antagonist ICI182780. 4-(tert-octyl)phenol also caused reductions in these parameters, but the effects were not abolished by ICI182780. Assay of S-COMT protein levels represents a simple and convenient method of assessing the estrogenic potential of a compound.
Export Options
About this article
Cite this article as:
Ho W.L. P., Chu C.Y. A., Kwok H.H. K., Liu F. H., Kung H.W. M., Ramsden B. D. and Ho L. S., Effects of Plasticisers and Related Compounds on the Expression of the Soluble Form of Catechol-O-Methyltransferase in MCF-7 Cells, Current Drug Metabolism 2008; 9 (4) . https://dx.doi.org/10.2174/138920008784220628
DOI https://dx.doi.org/10.2174/138920008784220628 |
Print ISSN 1389-2002 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5453 |
Call for Papers in Thematic Issues
Interaction between drugs and endocrine diseases
The introduction of highly active antiretroviral therapy accelerated studies and our understanding on the interaction between pharmacological therapies and endocrine diseases. Drugs can precipitate endocrine via different mechanisms, including direct alteration of hormone production and secretion, dysregulation of hormonal axis, effects on hormonal transport, receptor-binding, and cellular signalling. Common drug-induced ...read more
Tissue Distribution and Metabolism of Micro- and Nanoparticles and Medical Implants
With the continuous advancement of modern science and engineering, numerous functional materials and active molecules have been developed and utilized in various industrial, medical, and food applications. Many of these can enter the body, either actively or passively, and have significant and intricate impacts on human health. For example, biomaterials ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Angiotensin-Receptor Blockers and the Risk of Alzheimer´s Disease: A Meta-analysis
Current Reviews in Clinical and Experimental Pharmacology Role of Lycopene in the Control of ROS-Mediated Cell Growth: Implications in Cancer Prevention
Current Medicinal Chemistry Targeting Chromatin Remodeling to Prevent Cardiovascular Disease in Diabetes
Current Pharmaceutical Biotechnology The Therapeutic Potential of Melatonin in Neurological Disorders
Recent Patents on Endocrine, Metabolic & Immune Drug Discovery Drug Treatment of Metabolic Syndrome
Current Clinical Pharmacology Microdialysis Approach to Study Serotonin Outflow in Mice Following Selective Serotonin Reuptake Inhibitors and Substance P (Neurokinin 1) Receptor Antagonist Administration: A Review
Current Drug Targets Can the Electrophysiological Action of Rosiglitazone Explain its Cardiac Side Effects?
Current Medicinal Chemistry Association of Newly Diagnosed Hypertension and Polypharmacy with Frailty in a Tertiary Hospital Patients from Maracaibo City, Venezuela
Current Hypertension Reviews Management of Hypertension in Relation to Acute Coronary Syndromes and Revascularisation
Current Pharmaceutical Design Critical Role of the Endocannabinoid System in the Regulation of Food Intake and Energy Metabolism, with Phylogenetic, Developmental, and Pathophysiological Implications
Endocrine, Metabolic & Immune Disorders - Drug Targets Oxidative Stress Modulation Through Habitual Physical Activity
Current Pharmaceutical Design Metabolic and Cardiovascular Actions of Nesfatin-1: Implications in Health and Disease
Current Pharmaceutical Design Transmembrane Protein 166 and its Significance
Protein & Peptide Letters Copper and Neurotoxicity in Autism Spectrum Disorder
Current Pharmaceutical Design Subject Index To Volume 3
Vascular Disease Prevention (Discontinued) Endotoxin, TLR4 Signaling and Vascular Inflammation: Potential Therapeutic Targets in Cardiovascular Disease
Current Pharmaceutical Design Propionate: Hypophagic Effects Observed in Animal Models Might be Transposed to the Human Obesity Management
Current Nutrition & Food Science The Epidemiology and Health Effects of Tobacco Use
Current Pediatric Reviews What Makes a Pluripotency Reprogramming Factor?
Current Molecular Medicine An Integrated Computational Approach for Plant-Based Protein Tyrosine Phosphatase Non-Receptor Type 1 Inhibitors
Current Computer-Aided Drug Design