Effects of Plasticisers and Related Compounds on the Expression of the Soluble Form of Catechol-O-Methyltransferase in MCF-7 Cells

Author(s): P. W.L. Ho, A. C.Y. Chu, K. H.H. Kwok, H. F. Liu, M. H.W. Kung, D. B. Ramsden, S. L. Ho.

Journal Name: Current Drug Metabolism

Volume 9 , Issue 4 , 2008


Abstract:

Previously we have shown that E2 down regulates S-COMT expression. Here the effects of four phthalate esters and 4-(tertoctyl) phenol on the intra-cellular levels of S-COMT and COMT activity were studied in MCF-7 cells as a measure of estrogenic activity of these compounds. The four phthalate esters caused significant reductions in both S-COMT protein and COMT activity levels. These effects were inhibited by the ERα receptor antagonist ICI182780. 4-(tert-octyl)phenol also caused reductions in these parameters, but the effects were not abolished by ICI182780. Assay of S-COMT protein levels represents a simple and convenient method of assessing the estrogenic potential of a compound.

Keywords: COMT, plasticiser, phthalate, endocrine disruption, estrogen

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Article Details

VOLUME: 9
ISSUE: 4
Year: 2008
Page: [276 - 279]
Pages: 4
DOI: 10.2174/138920008784220628
Price: $58

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