Combinatorial Chemistry & High Throughput Screening

Rathnam Chaguturu 
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Retroviral Display and High Throughput Screening

Author(s): Christian J. Buchholz, Lydia J. Duerner, Sabrina Funke and Irene C. Schneider

Affiliation: Division of Medical Biotechnology, Paul-Ehrlich-Institut, Paul-Ehrlich-Str. 51-53, D-63225 Langen, Germany.

Keywords: Directed evolution, antibody, antigen, protease substrate, retroviral vector, protein engineering

Abstract:

Retroviruses distinguish themselves from all other mammalian viruses by their abilities to infect and propagate in mammalian cells without causing a cytopathic effect and to stably integrate their genetic information into the genome of the host cell. These unique properties make them an ideal platform for the display and directed evolution of proteins in a mammalian cell environment. This review will describe the essentials about retrovirus biology and then discuss in detail display and screening strategies that have been developed during the past 15 years of retroviral display technology.

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Article Details

VOLUME: 11
ISSUE: 2
Page: [99 - 110]
Pages: 12
DOI: 10.2174/138620708783744543