Current Pharmaceutical Biotechnology

Zeno Foldes-Papp
Visiting Professor of Medical Biochemistry
HELIOS Clinical Center of Emergency Medicine
Department for Internal Medicine
Alte-Koelner-Strasse 9
D-51688 Koeln-Wipperfuerth
Germany

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Encapsulating DNA within Biodegradable Polymeric Microparticles

Author(s): Corinne S. Lengsfeld, Mark C. Manning, Theodore W. Randolph.

Abstract:

In order for genetic medicines to become viable commercial products, the active form of the drug (e.g., DNA) must be able to reach the site of action and remain there long enough to accomplish its intended function. Encapsulation of plasmid DNA into biodegradable microspheres is one approach towards solving this challenge. This review describes the primary methods for satisfactorily entrapping intact DNA into biodegradable polymeric matrices. In particular, the materials, processes, and equipment required for each encapsulation method are described in detail. The resulting microspheres could be used for parenteral, oral, and inhalation therapy.

Keywords: encapsulating dna, microparticles, genetic medicines, polymeric matrices, inhalation therapy, transfection, oral delivery, genetic vaccines, cryopreparation

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Article Details

VOLUME: 3
ISSUE: 3
Year: 2002
Page: [227 - 235]
Pages: 9
DOI: 10.2174/1389201023378300
Price: $58