Automated Acquisition of Aptamer Sequences

Title: Automated Acquisition of Aptamer Sequences

Volume: 5 Issue: 4

Author(s): J. Colin Cox, Manjula Rajendran, Timothy Riedel, Eric A. Davidson, Letha J. Sooter, Travis S. Bayer, Mary Schmitz-Brown and Andrew D. Ellington

Affiliation:

Abstract: While the in vitro selection of nucleic acid binding species (aptamers) requires numerous liquidhandling steps, these steps are relatively straightforward and the overall process is therefore amenable to automation. Here we demonstrate that automated selection techniques are capable of generating aptamers against a number of diverse protein targets. Automated selection techniques can be integrated with automated analytical methods, including sequencing, determination of binding constants, and structural analysis. The methods that have so far been developed can be further multiplexed, and it should soon be possible to attempt the selection of aptamers against organismal proteomes or metabolomes.

Cite this article as:

J. Colin Cox , Manjula Rajendran , Timothy Riedel , Eric A. Davidson , Letha J. Sooter , Travis S. Bayer , Mary Schmitz-Brown and Andrew D. Ellington , Automated Acquisition of Aptamer Sequences, Combinatorial Chemistry & High Throughput Screening 2002; 5 (4) . https://dx.doi.org/10.2174/1386207023330291