Design and Peptide-Based Validation of Phage Display Antibodies for Proteomic Biochips

Author(s): N. Stich, G. van Steen, T. Schalkhammer.

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 6 , Issue 1 , 2003

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Abstract:

To validate potential application of phage display-antibody arrays for high-throughput screening on a novel proteomics biochip, we examined the epitopes versus the full protein of glucose-6-phosphatedehydrogenase (G6PD) from yeast. In a predictive approach, we used the Hopp-Woods method and compared the results with antibodies directed against the entire enzyme. In total, 16 peptides of a length of 11 amino acids each fulfilling the desired criteria were identified and synthesized. Subsequently, antibodies against G6PD were raised using a phage display library. Selective interaction of the antibodies with certain peptides facilitated the identification of epitopes predicted by the hydropathic profile. The setup was adapted to a novel biochip system based on surface-enhanced absorption for direct CCD-camera based screening.

Keywords: epitope prediction, peptide synthesis, phage display antibody library, biochip, nanotechnology, proteomics

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Article Details

VOLUME: 6
ISSUE: 1
Year: 2003
Page: [67 - 78]
Pages: 12
DOI: 10.2174/1386207033329841

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