Metal-Enhanced Fluorescence: Potential Applications in HTS
Chris D. Geddes, Ignacy Gryczynski, Joanna Malicka, Zygmunt Gryczynski and Joseph R. Lakowicz
Affiliation: Center for Fluorescence Spectroscopy and Institute of Fluorescence, University of Maryland Biotechnology Institute, Medical Biotechnology Center, 725 West Lombard Street, Baltimore, Maryland 21201, USA
Keywords: Metal-Enhanced, Fluorescence, photostability, biochemical species
Metallic surfaces and particles can have dramatic effects on fluorescence, including localized excitation, increased quantum yields, increased photostability and increased distances for resonance energy transfer (RET), and directional emission. While all these effects have not yet been realized in a single system, metal-enhanced fluorescence promises to provide the next generation of high sensitivity fluorescence assays for low copy number detection of biochemical species.
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