Fluorescence Assays for High-Throughput Screening of Protein Kinases

Author(s): G. J.R. Zaman, A. Garritsen, Th. de Boer, C. A.A. van Boeckel.

Journal Name: Combinatorial Chemistry & High Throughput Screening

Volume 6 , Issue 4 , 2003

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Protein kinases comprise one of the most important group of targets for drug discovery research today. Methods to identify novel kinase inhibitors by high-throughput screening have evolved rapidly in recent years. An important aspect is the availability of fluorescent probes that can be applied in a homogeneous, or mix-and-measure, assay format. Here, we illustrate the application of fluorescence read-out technologies for kinase targets in light of our own experiences in assay development and high-throughput screening.

Keywords: fluorescence assays, high-throughput screening, protein kinases, novel kinase inhibitors

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Article Details

Year: 2003
Page: [313 - 320]
Pages: 8
DOI: 10.2174/138620703106298563
Price: $58

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