A Fluorescent High-Throughput Assay for Double-Stranded DNA: the RediPlate PicoGreen Assay

Author(s): Robert Batchelor, David Hagen, Iain Johnson, Joseph Beechem.

Journal Name: Combinatorial Chemistry & High Throughput Screening

Volume 6 , Issue 4 , 2003

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Abstract:

The fluorescent PicoGreen® reagent for detection and quantitation of double-stranded DNA has been adapted for high-throughput screening: the RediPlate PicoGreen double-stranded DNA assay format. In the RediPlate PicoGreen assay format, the PicoGreen reagent is predistributed and co-dried into either 96- or 384- well microplates with the excipient trehalose. The user resuspends the dried reagents upon adding DNA, and measures the resulting fluorescence after a five minute incubation. Replicate fluorescence measurements on nominally identical wells have less than a 5% coefficient of variation. The assay is linear from 5 to 500 ng / ml DNA in a 200 μl volume. The RediPlate PicoGreen assay format retains the advantages of the original PicoGreen reagent - sensitivity, speed, and specificity - but in a high-throughput format.

Keywords: high-throughput, fluorescence, dna, microplate, dye-binding assay, contaminant tolerance

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Article Details

VOLUME: 6
ISSUE: 4
Year: 2003
Page: [287 - 291]
Pages: 5
DOI: 10.2174/138620703106298536
Price: $58

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