Optimal Enzymes for Single-Molecule Sequencing

Author(s): Susanne Brakmann .

Journal Name: Current Pharmaceutical Biotechnology

Volume 5 , Issue 1 , 2004

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Abstract:

Much research effort has been made to realize a single-molecule sequencing. Central to this project are two enzymatic tasks that challenged several biochemists during the last years. They searched for polymerases for copying a DNA target into completely fluorophore-labeled DNA as well as for handling this new DNA derivative. Furthermore, they studied exonucleases for the sequential hydrolysis of completely dye-labeled DNA and explored optimal conditions for future application on the single-molecule level. In this article, the recent advances will be summarized from the biochemists point of view.

Keywords: dna sequencing, single-molecule detection, dna polymerase, exonuclease, fluorescence

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Article Details

VOLUME: 5
ISSUE: 1
Year: 2004
Page: [119 - 126]
Pages: 8
DOI: 10.2174/1389201043489611

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