Abstract
Immunoassays, or assays that are using antibodies as the specific binding reagents, have become one of the most common methodologies in diagnostic laboratories. In this paper we review different configurations of immunoassays as applied to a variety of analytes and sensitivity limits, along with common detection techniques and strategies. Progress in developing of ultra high affinity antibodies as a direction to improved immunoassays is also reviewed. Finally, we specifically concentrate on determination of antibody binding constants and performing immunoassays at the single molecule level using Fluorescence Correlation Spectroscopy (FCS). This technique has become a powerful tool in molecular binding characterizations and assay development, and possibly will grow into a quantitative analytical method suitable for diagnostic tests.
Keywords: Antibodies, immunoassays, Fluorescence, Spectroscopy
Current Pharmaceutical Biotechnology
Title: Antibodies in Diagnostic Applications
Volume: 5 Issue: 1
Author(s): Sergey Y. Tetin and Stephen D. Stroupe
Affiliation:
Keywords: Antibodies, immunoassays, Fluorescence, Spectroscopy
Abstract: Immunoassays, or assays that are using antibodies as the specific binding reagents, have become one of the most common methodologies in diagnostic laboratories. In this paper we review different configurations of immunoassays as applied to a variety of analytes and sensitivity limits, along with common detection techniques and strategies. Progress in developing of ultra high affinity antibodies as a direction to improved immunoassays is also reviewed. Finally, we specifically concentrate on determination of antibody binding constants and performing immunoassays at the single molecule level using Fluorescence Correlation Spectroscopy (FCS). This technique has become a powerful tool in molecular binding characterizations and assay development, and possibly will grow into a quantitative analytical method suitable for diagnostic tests.
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Cite this article as:
Tetin Y. Sergey and Stroupe D. Stephen, Antibodies in Diagnostic Applications, Current Pharmaceutical Biotechnology 2004; 5 (1) . https://dx.doi.org/10.2174/1389201043489602
DOI https://dx.doi.org/10.2174/1389201043489602 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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