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Current Molecular Medicine
ISSN (Print): 1566-5240
ISSN (Online): 1875-5666
VOLUME: 4
ISSUE: 7
DOI: 10.2174/1566524043359944      Price:  $58









Inflammatory Modulation of Hepatocyte Apoptosis by Nitric Oxide: In Vivo, In Vitro, and In Silico Studies

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Author(s): Yoram Vodovotz, Peter K. M. Kim, Elife Zerrin Bagci, G. Bard Ermentrout, Carson C. Chow, Ivet Bahar and Timothy R. Billiar
Pages 753-762 (10)
Abstract:
Nitric oxide (NO.) and its reaction products are key players in the physiology and pathophysiology of inflammatory settings such as sepsis and shock. The consequences of the expression of inducible NO. synthase (iNOS, NOS-2) can be either protective or damaging to the liver. We have delineated two distinct hepatoprotective actions of NO.: the stimulation of cyclic guanosine monophosphate and the inhibition of caspases by S-nitrosation. In contrast, iNOS / NO. promotes hepatocyte death under conditions of severe redox stress, such as hemorrhagic shock or ischemia / reperfusion. Redox stress activates an unknown molecular switch that transforms NO., which is hepatoprotective under resting conditions, into an agent that induces hepatocyte death. We hypothesize that the magnitude of the redox stress is a major determinant for the effects of NO. on cell survival by controlling the chemical fate of NO.. To address this hypothesis, we have carried out studies in relevant in vivo and in vitro settings. Moreover, we have constructed an initial mathematical model of caspase activation and coupled it to a model describing some of the reactions of NO • in hepatocytes. Our studies suggest that modulation of iron, oxygen, and superoxide may dictate whether NO. is hepatoprotective or hepatotoxic.
Keywords:
inflammatory inos, isoform, gene transcription, cytokines, apoptosis, rodent models, inflammation
Affiliation:
Department of Surgery,University of Pittsburgh, Montefiore Hospital, NW653, 3459 Fifth Ave.Pittsburgh, PA 15213, USA.