Towards Sorting of Biolibraries Using Single-Molecule Fluorescence Detection Techniques

Author(s): Antonie J.W.G. Visser, Beno H. Kunst, Hans Keller, Arjen Schots.

Journal Name: Current Pharmaceutical Biotechnology

Volume 5 , Issue 2 , 2004

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Abstract:

The selection of specific binding molecules like peptides and proteins from biolibraries using, for instance, phage display methods can be quite time-consuming. It is therefore desirable to develop a strategy that is much faster in selection and sorting of potential binders out of a biolibrary. In this contribution we separately discuss the current achievements in generation of biolibraries, single-molecule detection techniques and microfluidic devices. A highthroughput microfluidic platform is then proposed that combines the propulsion of liquid containing fluorescent components of the biolibrary through microchannels, single-molecule fluorescence photon burst detection and real-time sorting of positive hits.

Keywords: biolibrary, phage display, selection, sorting

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Article Details

VOLUME: 5
ISSUE: 2
Year: 2004
Page: [173 - 179]
Pages: 7
DOI: 10.2174/1389201043376977

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