Revisiting Pitfalls, Problems and Tentative Solutions for Assaying [General Articles] Mitochondrial Respiratory Chain Complex III in Human Samples

Author(s): Dominique Chretien, Abdelhamid Slama, Jean-Jacques Briere, Arnold Munnich, Agnes Rotig, Pierre Rustin.

Journal Name: Current Medicinal Chemistry

Volume 11 , Issue 2 , 2004

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Abstract:

The assessment of mitochondrial respiratory chain enzyme activity in human samples is a difficult task due to both the small amount of tissue generally available and the frequent need to perform enzyme activity measurement in crude mitochondrial fraction. This is particularly true for the measurement of complex III activity which partial deficiency can be easily overlooked. In this review, we first consider the several interfering reactions occurring when measuring this activity. We subsequently describe the use of an alkyl glycoside detergent, lauryl maltoside, to keep these interfering reactions to a very low level. Next, we quantify the effect of the detergent on the actual measurement of complex III in various human tissue samples and cells. Finally, we also demonstrate that the use of the detergent allows (i) a better detection of an inherited partial defect affecting cytochrome b, a catalytic subunit of the mitochondrial complex III, (ii) to possibly discriminate decreased complex III activity resulting from an abnormal complex III assembly (BCS1 gene mutation) from an hampered catalytic activity originating from a cytochrome b mutation. This detailed review of the problems associated with complex III assessment and of their tentative solution highlights the difficulties still encountered in the measurements of mitochondrial respiratory chain in humans.

Keywords: respiratory chain, mitochondrial disease, complex III, lauryl maltoside, bcs1, cytochrome b, quinone

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Article Details

VOLUME: 11
ISSUE: 2
Year: 2004
Page: [233 - 239]
Pages: 7
DOI: 10.2174/0929867043456151
Price: $58